rabbit anti pstat3 y705  (Cell Signaling Technology Inc)

Journal: Breast Cancer Research : BCR

Article Title: Divergent paths of mammary gland involution: unveiling the cellular dynamics in abruptly and gradually involuted mouse models

doi: 10.1186/s13058-024-01933-3

Figure Lengend Snippet: Expression pattern of acute phase response genes is distinct in AI and GI glands. Quantitative RT-PCR analysis of A. Stat3 , B. C/EBPδ , C. CtsL , D. CtsB , E. Orm1 , F. Orm2 , G. Lrg1 , H. Slpi , I. CD14 , J. Chi3L1, and K. MMP2 . Expression normalized to RPL37 . n = 3 per group per time point. Li. Western blot analysis and quantitation of Lii. pStat3 Y705 , Liii. Stat3, Liv. CtsB, and Lv. CD14. M. Temporal pattern of pSTAT3 Y705 positive cell distribution in AI and GI glands between day 7 and day 28 postpartum. n = 6 per group per time point. Ni. Representative images of FFPE sections of AI (day 11 PPM) and GI (day 22 and 25 PPM) mammary glands subjected to immunofluorescence analysis of pStat3 Y705 (green) and CD14 (red). DAPI (blue) was used to counterstain the nuclei. Nii. Bar diagram showing the number of pStat3. Y705 and CD14 double-positive cells per field of view (FOV) in mammary gland sections of AI (day 11 PPM) and GI (day 22 and 25 PPM). n = 5 per group per time point. Number of FOV = 10

Article Snippet: Primary antibodies used for IHC and IF were anti-pStat3 Y705 (1:100, #9145, Cell Signaling Technology, USA), CD14 (1:50, #60253–1, Invitrogen, USA) and anti-F4/80 (1:500, MF48000, Invitrogen, USA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Quantitation Assay, Immunofluorescence